11 Common Lab Mistakes To Avoid

11 Common Lab Mistakes To Avoid

Working in a research laboratory takes a great deal of effort and focus. Yes, it comes with its share of failures and frustrations. However, with hard work and dedication, you can achieve success and reduce the rate of failures.

Unfortunately, lack of information, lack of expertise, and mistakes are quite common. What we make in the process are some of the frequent causes that cause most lab failures. Of all, we all make mistakes; it’s all part of the learning process. But, the sooner we recognize and correct the error, the sooner we will be able to succeed. Moreover, conduct a more perfect experiment in the future. In this article, we have gathered 11 common mistakes that you should avoid when working in Labs.

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1. Not Dissolving Your Drugs/reagents

For example, you are a researcher at a lab in Houston. While you test your drugs, reagents may not fully dissolve. It needs to be at/ taking the medication at the optimum concentration. The solution is to study your product sheet determining what to do, warming or sonicating. But don’t forget to use your eyes and bring your sample up to the light to make sure it’s completely dissolved.

2. Ordering the Wrong Product

Supplier catalogs frequently include a confusing assortment of chemical substances, reagents, and variations. Which you must traverse. From purchasing the incorrect enantiomer of a receptor ligand to ordering reverse complement primers by mistake. The repercussions of making a bad purchase are obvious: time and money are wasted. And if the error isn’t discovered before the experiment begins, your experiment will be of no use.

3. Using the Wrong Reagent in Your Experiment

When producing a polyacrylamide gel. Our panel of researchers used 2-mercaptoethanol instead of TEMED. Since they didn’t read the container labels properly, they ended up using the wrong secondary antibody along with incorporating the wrong chemicals. These kinds of errors have far-reaching implications. Necessitating a rerun of the experiment or procedure.

4. Running Your Electrophoresis Backward!

This one has to do with performing Western Blots. If you reverse the connection wires on the power supply. Then run the electrophoresis backward, your samples will be lost. It’s a blunder that even the most seasoned scholars may commit.

5. Using Wrong Statistical Test

Using the incorrect statistical analysis on data is a costly error. That might take a long time to correct. You may wind up misinterpreting your data. Only when you base your findings on an incorrect interpretation of your results. Furthermore, if your experiment differs from your lab colleague’s.

The statistical test assistants or technicians ‘ performance may not be acceptable for you. The solution is to choose your technique of analysis at the planning stage. For Instance, if any researcher at laboratories in Houston TX isn’t a statistical specialist, they should double-check with their supervisors. So, you know you’ve chosen the proper one.

6. Losing Your Peptides!

When you’re done with freeze-drying your peptides, slowly open the and release valve.

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7. Insufficient Centrifugation of Samples

A typical error is not spinning solubilized materials fast before fusion takes place. Non-solubilized membrane components and protein clumps will remain in the sample. If the centrifugation force is not strong enough. In co-immunoprecipitation and GST pull-down studies, these will be designated as “interaction partners.” It’s also advised to look out for when reading scientific papers. Make sure the approach utilizes an adequate centrifuge, not a low-speed one. Because low-speed centrifuges aren’t capable of completely removing insoluble protein clumps.

8. Poor or Mislabeling of Samples

If your samples become mixed up, you’ll have to restart your experiment from the beginning. If you’re handwriting labels, make sure they’re readable. And if you’re using a physical label (rather than writing on your vial), choose one that’s right for you. Such as one that can endure a freezer or a water bath. If you’re working in a lab in Houston, as always, be consistent in your labeling. Stick to your lab regulations, and avoid any misunderstandings.

9. Not Filling Your Centrifuge Tubes Up Enough

Another common centrifuge blunder is failing to fill your ultracentrifuge tubes with liquid. If you leave the tubes half-filled, they will quickly collapse.

10. Not Paying Attention to Detail

The key to a successful experiment is to follow set protocol daily and precisely. Paying attention to solution flow rates. Maintaining a steady recording baseline and designing efficient stimulating electrodes. For example, all contribute to a successful result in the electrophysiology lab in Houston.

11. Using Lab Coats Anywhere Other Than Lab

The purpose of lab coats is to keep the unpleasant and hazardous substances out of our garments. As a result, it’s self-evident that lab coats are full of undesirables. Yet, some people become lazy or forget to take them off when they need to go somewhere other than the lab. They continue to wear them whether they are going for tea, coffee, or restroom breaks. This allows them to spread these germs into the environment. As well as bring harmful external agents into the lab that may interfere with the studies. As a result, this is a terrible idea, especially if you’re working in biological labs.

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Conclusion

Finally, failing to follow appropriate laboratory procedures leads to errors. While the majority of us are decent citizens who strive to follow. and advocate for these practices. We fail to follow our laboratory safety officers’ instructions which are for our own safety. That’s a major error, we can’t afford to incur if we want to keep ourselves and others safe at work. Shortcuts should be avoided. If the incubation duration is 30 minutes, be patient. Wait until the incubation period is over before proceeding to the next step. When you’re planning an experiment, figure out how much time you’ll need. Making a mistake in experimental research should always be taken seriously. But it should not discourage researchers. You can avoid all these errors if researchers are well trained and watchful. We not only create a safer, more productive workplace. But we also perform better science as a result of minimizing errors.

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